Rhizosphere soil samples were preferred as it contains most of the prospective antibiotic producers. In an attempt to screen out new potent antibiotic producers from soil, Rhizobium species wan isolated. Antibiotic produced by shake flask culture is found to be effective against both gram positive and gram negative organism. This product was found to be very promising antibiotics when assayed biologically. UV treatment showed positive effect on antibiotic production. The molecular structure of antibiotic was identified by using advanced analytical techniques like UV Visible spectroscopy, FTIR, GCMS, NMR spectroscopy.
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